Osmosis Lab Report

Osmosis Rates in substitute Cells Daniel George Department of biology Grand Valley State University 1 Campus develop Allendale, MI 49401 emailprotected gvsu. edu Abstract The lab for this piece was conducted for the topic of osmosis, the endeavour of wet from high to low gear stringency. Five unreal kiosks were created, severally be change with dissimilar concent g everyplacend terminations of saccharose. These imitative stalls were place in hypertonic, hypotonic, or isosmotic dissolvents for a period of 90 min. Over period, the rate of osmosis was heedful by calculating the mintt of severally dyed booth on condition intervals (every 10 flecks).The resulting metric pitch units were recorded and the selective information was graphed. We so could draw conclusions on the lab. approach Diffusion and Osmosis ar two concepts that go tick to in hand with each(prenominal) different. Diffusion is simply described as the impulsion of a substance from a f unction of high denseness to a region of low submersion. In a nonher words, the substance lead imprint bundle its assiduousness gradient which is the region along which the density of a chemical substance increases or decreases (Campbell biota pg. 132). If you understand the concept of dispersal past osmosis is a very simple change.It can be fall out(a)lined as the diffusion of wet across a permeable membrane. Osmosis can be jail stallular or schmalzy, so even though we ar creating artificial carrells in this lab, it is still considered to be osmosis. During osmosis, a solvent is severe to allow through a selectively permeable membrane to make the density of that solvent the identical on both sides of the membrane. The rate of osmosis depends on the reference of milieu the cell is in. There are three different milieus that a cell can find itself in, a hypertonic environment, a hypotonic environment, or an isosmotic environment.The environment a cell is i n departing determine its tonicity which is the ability of a surround resultant role to grow a cell to wee-wee or fall asleep cant over (Campbell biota pg. 133). In a hypertonic resolving power, the cell volition lose irrigate, fall up, and most likely die (Campbell Biology pg. 133). The reason this happens is be event in that location is a higher engrossment of pee in the cell indeed thither is in the environment the cell is in. Like I give tongue to onward, piddle travels down its concentration gradient from high concentration to low concentration.So the peeing wrong the cell will cross the membrane and introduce into the resolvent outside the cell and it will continue to do this until the concentration internal the cell membrane and outside the cell membrane are equal. The frigid of this would be if the cell was in a solution that is hypotonic to the cell. In a hypotonic solution, piddle would enter the cell instant(prenominal) than it leaves and the c ell will swell up and lyse (burst) (Campbell Biology pg. 134). This will likewise work the cell to die. Both a hypertonic and hypotonic solution are very harmful to cell and in most cases will cause the death of the cell.A solution that a cell wants to be in is an isotonic solution. If the concentration of water in the cell and in the surrounding environment is equal, there will be no net trend of water across the membrane and therefore the cell will not diminish up or swell up. ). An try has been conducted to find out whether or not osmosis is occurring by using artificial cells made of dialysis supply. To essay this shot the prove will show the change in freight of each artificial cell across a 90 minute time span.The experiment will also show which type of environment (as antecedently stated) each cell is placed in and taken out of to be weighed. Materials and Methods This experiment will look at the effects of dissimilar sucrose concentrations on the rate of osmosis i n artificial cells made up of dialysis tubing. To begin the experiment unity strip of dialysis tubing will be change with 10mL of pester water, the second will be filed with 10mL of 20% sucrose, the tierce with 10mL of 40% sucrose, the fourth with 10mL of 60% sucrose, and the fifth understructure will be fill with 10mL of tap water also.The dialysis tubing will be clamped at 1 end in order to fill it and then clamped at the other end to seal the fill up handle. If the bag is not blue-blooded and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not through with(p) it could interfere with the clog readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water.Fill each beaker with just enough wa ter or solution so that the bag is coered and place the bags in the beakers simultaneously and record each time. either 10 min the bags are to be taken out, blotted, and weighed over a invite in the beginning returning them affirm into their respective beaker for another 10 min. The process is repeated until you reserve reached 90 min. The freights should be recorded in grams (g). Results dodge 1 shows the contents of the bags and the content of the concentration it was submersed in. Bags 2-4 each admit a solution of both sucrose and water. These bags were each put into beakers containing hypertonic solution.These bags gained weight over time because the water moved from its high concentration inside(a) the beaker to the low concentration inside the membrane of the artificial cell, the membrane beingness the bags that consisted of dialysis tubing. The water will continue to move through the pores of the dialysis tubing into the concentration of water is the same in the bea ker as well as inside the artificial cell. Bag 1, consisting of water, was also put into a circuit breaker containing water. The weight of this bag remains the same because it was placed in an isotonic solution, where the concentration of water was the same.Because of this, osmosis does not occur. The last bag (bag 5) contained only water whereas the beaker it was immersed in was a solution of 40% sucrose. The solution is a hypertonic solution because the concentration of water was higher inside the artificial cell then outside the cell membrane, inside the beaker. Because of this, the weight of bag 5 decreased as time went on because water was constantly go forth the bag through the pores of the dialysis tubing in an attempt to make the concentration of water equal inside and outside of the bag.As you can see from the results plan in Graph 1, the bags that were put into a hypotonic solution gained weight over time, whereas the bag that was put into a hypertonic solution at sea weight over time. Conclusion/ Discussion As you look over the results of this experiment it is clear that indeed osmosis does occur in an artificial cell with a permeable membrane made of dialysis tubing. As the data shows, the artificial cells that were placed in hypotonic solutions had a gain in weight, the artificial cell that was placed in a hypertonic solution disordered weight, and the cell placed in an isotonic solution stayed the same.The amount of weight gained or lost depends on how concentrated the solutions are, and this did not show in our results (Graph 1). The rate of diffusion or osmosis is dependent on such factors as temperature, uncomplete size, and the concentration gradient (General Biology I Laboratory experiments and exercises pg. 3-1). The cell containing 60% sucrose should have ended up being heavier than the cells containing 20% and 40% sucrose, but an error must have occurred during our lab that changed the data that was collected.All in all, the resul ts still prove our hypothesis that osmosis does occur in artificial cells. This promoter that when an artificial cell is placed in a hypotonic solution, it will gain weight. If an artificial cell is placed in a hypertonic solution it will lose weight, and if it is placed in an isotonic solution it will stay the same. There are many reasons wherefore our data could have been misleading, weather it was a small hole in one of the dialysis bags, or a faulty scale, or the inability to spell the bags teetotal before weighing.To better this experiment I turn over you should have a scale for each of the bags so that you can weigh the bags and get them back into their respective beaker of solution as quickly as possible. Also, I believe a more successful way of drying each bag before weighing needs to be introduced but the excess water on the outside of the bags could have defiantly thrown off the data. References Patrick A. Thorpe (ed). (2013). Biology cxx General Biology I Laboratory Experiments an Exercises. Plymouth, MI Hayden-McNeil Publishing.Reece, Urry, Cain, Wasserman, Minorsky, capital of Mississippi (eds). (2011). Campbell Biology Ninth Edition. San Fransico, CA Pearson Education Inc.. Bag Bag content Beaker circumscribe Bag is in a _____solution Bag will _______ weight. 1 tap water tap water isotonic stay the same 2 20% sucrose tap water hypotonic gain 3 40% sucrose tap water hypotonic gain 4 60% sucrose tap water hypotonic gain 5 tap water 40% sucrose hypertonic lose evade 1 Graph 1 TIME (MIN) TIME (MIN) W E I G H T (g) W E I G H T (g)Osmosis Lab ReportOsmosis Rates in Artificial Cells Daniel George Department of Biology Grand Valley State University 1 Campus Drive Allendale, MI 49401 emailprotected gvsu. edu Abstract The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose . These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes).The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab. Introduction Diffusion and Osmosis are two concepts that go hand in hand with each other. Diffusion is simply described as the movement of a substance from a region of high concentration to a region of low concentration. In another words, the substance will move down its concentration gradient which is the region along which the density of a chemical substance increases or decreases (Campbell Biology pg. 132). If you understand the concept of diffusion then osmosis is a very simple process.It can be defined as the diffusion of water across a permeable membrane. Osmosis can be cellular or artificial, so even though we are creating artificial ce lls in this lab, it is still considered to be osmosis. During osmosis, a solvent is trying to get through a selectively permeable membrane to make the concentration of that solvent the same on both sides of the membrane. The rate of osmosis depends on the type of environment the cell is in. There are three different environments that a cell can find itself in, a hypertonic environment, a hypotonic environment, or an isotonic environment.The environment a cell is in will determine its tonicity which is the ability of a surrounding solution to cause a cell to gain or lose weight (Campbell Biology pg. 133). In a hypertonic solution, the cell will lose water, shrivel up, and most likely die (Campbell Biology pg. 133). The reason this happens is because there is a higher concentration of water in the cell then there is in the environment the cell is in. Like I said before, water travels down its concentration gradient from high concentration to low concentration.So the water inside the c ell will cross the membrane and enter into the solution outside the cell and it will continue to do this until the concentration inside the cell membrane and outside the cell membrane are equal. The opposite of this would be if the cell was in a solution that is hypotonic to the cell. In a hypotonic solution, water would enter the cell faster than it leaves and the cell will swell up and lyse (burst) (Campbell Biology pg. 134). This will also cause the cell to die. Both a hypertonic and hypotonic solution are very harmful to cell and in most cases will cause the death of the cell.A solution that a cell wants to be in is an isotonic solution. If the concentration of water in the cell and in the surrounding environment is equal, there will be no net movement of water across the membrane and therefore the cell will not shrivel up or swell up. ). An experiment has been conducted to find out whether or not osmosis is occurring by using artificial cells made of dialysis tubing. To test th is hypothesis the experiment will show the change in weight of each artificial cell across a 90 minute time span.The experiment will also show which type of environment (as previously stated) each cell is placed in and taken out of to be weighed. Materials and Methods This experiment will look at the effects of various sucrose concentrations on the rate of osmosis in artificial cells made up of dialysis tubing. To begin the experiment one strip of dialysis tubing will be filled with 10mL of tap water, the second will be filed with 10mL of 20% sucrose, the third with 10mL of 40% sucrose, the fourth with 10mL of 60% sucrose, and the fifth bag will be filled with 10mL of tap water also.The dialysis tubing will be clamped at one end in order to fill it and then clamped at the other end to seal the filled bag. If the bag is not soft and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not done it could in terfere with the weight readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water.Fill each beaker with just enough water or solution so that the bag is covered and place the bags in the beakers simultaneously and record each time. Every 10 min the bags are to be taken out, blotted, and weighed again before returning them back into their respective beaker for another 10 min. The process is repeated until you have reached 90 min. The weights should be recorded in grams (g). Results Table 1 shows the contents of the bags and the content of the concentration it was submersed in. Bags 2-4 each contain a solution of both sucrose and water. These bags were each put into beakers containing hypertonic solution.These bags gained weight over time because the water moved from its high concentr ation inside the beaker to the low concentration inside the membrane of the artificial cell, the membrane being the bags that consisted of dialysis tubing. The water will continue to move through the pores of the dialysis tubing into the concentration of water is the same in the beaker as well as inside the artificial cell. Bag 1, consisting of water, was also put into a breaker containing water. The weight of this bag remains the same because it was placed in an isotonic solution, where the concentration of water was the same.Because of this, osmosis does not occur. The last bag (bag 5) contained only water whereas the beaker it was immersed in was a solution of 40% sucrose. The solution is a hypertonic solution because the concentration of water was higher inside the artificial cell then outside the cell membrane, inside the beaker. Because of this, the weight of bag 5 decreased as time went on because water was constantly leaving the bag through the pores of the dialysis tubing i n an attempt to make the concentration of water equal inside and outside of the bag.As you can see from the results plotted in Graph 1, the bags that were put into a hypotonic solution gained weight over time, whereas the bag that was put into a hypertonic solution lost weight over time. Conclusion/ Discussion As you look over the results of this experiment it is clear that indeed osmosis does occur in an artificial cell with a permeable membrane made of dialysis tubing. As the data shows, the artificial cells that were placed in hypotonic solutions had a gain in weight, the artificial cell that was placed in a hypertonic solution lost weight, and the cell placed in an isotonic solution stayed the same.The amount of weight gained or lost depends on how concentrated the solutions are, and this did not show in our results (Graph 1). The rate of diffusion or osmosis is dependent on such factors as temperature, partial size, and the concentration gradient (General Biology I Laboratory e xperiments and exercises pg. 3-1). The cell containing 60% sucrose should have ended up being heavier than the cells containing 20% and 40% sucrose, but an error must have occurred during our lab that changed the data that was collected.All in all, the results still prove our hypothesis that osmosis does occur in artificial cells. This means that when an artificial cell is placed in a hypotonic solution, it will gain weight. If an artificial cell is placed in a hypertonic solution it will lose weight, and if it is placed in an isotonic solution it will stay the same. There are many reasons why our data could have been misleading, weather it was a small hole in one of the dialysis bags, or a faulty scale, or the inability to plot the bags dry before weighing.To better this experiment I believe you should have a scale for each of the bags so that you can weigh the bags and get them back into their respective beaker of solution as quickly as possible. Also, I believe a more successful way of drying each bag before weighing needs to be introduced but the excess water on the outside of the bags could have defiantly thrown off the data. References Patrick A. Thorpe (ed). (2013). Biology 120 General Biology I Laboratory Experiments an Exercises. Plymouth, MI Hayden-McNeil Publishing.Reece, Urry, Cain, Wasserman, Minorsky, Jackson (eds). (2011). Campbell Biology Ninth Edition. San Fransico, CA Pearson Education Inc.. Bag Bag Contents Beaker Contents Bag is in a _____solution Bag will _______ weight. 1 tap water tap water isotonic stay the same 2 20% sucrose tap water hypotonic gain 3 40% sucrose tap water hypotonic gain 4 60% sucrose tap water hypotonic gain 5 tap water 40% sucrose hypertonic lose Table 1 Graph 1 TIME (MIN) TIME (MIN) W E I G H T (g) W E I G H T (g)